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Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells

Identifieur interne : 002519 ( Main/Exploration ); précédent : 002518; suivant : 002520

Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells

Auteurs : A. R. M. Townsend [Royaume-Uni] ; A. J. Mcmichael [Royaume-Uni] ; N. P. Carter [Royaume-Uni] ; J. A. Huddleston [Royaume-Uni] ; G. G. Brownlee [Royaume-Uni]

Source :

RBID : ISTEX:65CF7F75092E92EF09C74FAA9FF0FF4DDE625395

Descripteurs français

English descriptors

Abstract

Abstract: L cells expressing either the A/NT/60/68 nucleoprotein or the A/PR/8/34 (H1) hemagglutinin by DNA mediated gene transfer were used to investigate recognition by influenza A specific cytotoxic T lymphocytes (CTL). A subpopulation of CTL that recognized the H1 hemagglutinin was detected in mice primed with either A/PR/8/34 (H1N1) or A/JAP/305/57 (H2N2) influenza viruses. However, neither CTL from mice primed with A/NT/60/68 (H3N2) nor the recombinant virus X31 (H3N2) showed any activity on L cells expressing H1. These results showed that the majority of fully crossreactive CTL do not recognize the hemagglutinin molecule. A comparison between nucleoprotein and hemagglutinin transfected L cells reveals the nucleoprotein as the major target for CTL that are crossreactive on the three pandemic strains of human influenza A virus.

Url:
DOI: 10.1016/0092-8674(84)90187-9


Affiliations:


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<term>Immunoenzyme Techniques</term>
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<term>Bacterial gene</term>
<term>Berg</term>
<term>Binding affinity</term>
<term>Binding characteristics</term>
<term>Braciale</term>
<term>Brownlee</term>
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<term>Cell</term>
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<term>Cell clones</term>
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<term>Cell response</term>
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<term>First layer</term>
<term>First panel</term>
<term>Fluorescence profiles</term>
<term>Fourth panel</term>
<term>Further work</term>
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<term>Gene copies</term>
<term>Gene transfer</term>
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<term>Hemagglutinin</term>
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<term>Hemagglutinin gene</term>
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<term>Hemagglutinrn gene</term>
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<term>Human influenza</term>
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<term>Indirect immunoperoxidase staining</term>
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<term>Room temperature</term>
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<term>Scatchard analysis</term>
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<term>Specific lysis</term>
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<term>Unpublished data</term>
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<term>Viral</term>
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<term>Virus crossreactive</term>
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<div type="abstract" xml:lang="en">Abstract: L cells expressing either the A/NT/60/68 nucleoprotein or the A/PR/8/34 (H1) hemagglutinin by DNA mediated gene transfer were used to investigate recognition by influenza A specific cytotoxic T lymphocytes (CTL). A subpopulation of CTL that recognized the H1 hemagglutinin was detected in mice primed with either A/PR/8/34 (H1N1) or A/JAP/305/57 (H2N2) influenza viruses. However, neither CTL from mice primed with A/NT/60/68 (H3N2) nor the recombinant virus X31 (H3N2) showed any activity on L cells expressing H1. These results showed that the majority of fully crossreactive CTL do not recognize the hemagglutinin molecule. A comparison between nucleoprotein and hemagglutinin transfected L cells reveals the nucleoprotein as the major target for CTL that are crossreactive on the three pandemic strains of human influenza A virus.</div>
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