Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells
Identifieur interne : 002519 ( Main/Exploration ); précédent : 002518; suivant : 002520Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells
Auteurs : A. R. M. Townsend [Royaume-Uni] ; A. J. Mcmichael [Royaume-Uni] ; N. P. Carter [Royaume-Uni] ; J. A. Huddleston [Royaume-Uni] ; G. G. Brownlee [Royaume-Uni]Source :
- Cell [ 0092-8674 ] ; 1984.
Descripteurs français
- KwdFr :
- ADN (métabolisme), Animaux, Cellules cultivées, DNA restriction enzymes, Femelle, Lignées consanguines de souris, Lymphocytes T cytotoxiques (immunologie), Plasmides, Protéines de l'enveloppe virale (génétique), Protéines de la matrice virale, Protéines virales (génétique), Souris, Techniques immunoenzymatiques, Transcription génétique, Transfection, Virus de la grippe A (génétique), Virus de la grippe A (immunologie), Virus simien 40 (génétique).
- MESH :
- génétique : Protéines de l'enveloppe virale, Protéines virales, Virus de la grippe A, Virus simien 40.
- immunologie : Lymphocytes T cytotoxiques, Virus de la grippe A.
- métabolisme : ADN.
- Animaux, Cellules cultivées, DNA restriction enzymes, Femelle, Lignées consanguines de souris, Plasmides, Protéines de la matrice virale, Souris, Techniques immunoenzymatiques, Transcription génétique, Transfection.
English descriptors
- KwdEn :
- Animals, Cells, Cultured, DNA (metabolism), DNA Restriction Enzymes, Female, Immunoenzyme Techniques, Influenza A virus (genetics), Influenza A virus (immunology), L Cells (immunology), L Cells (metabolism), Mice, Mice, Inbred Strains, Plasmids, Simian virus 40 (genetics), T-Lymphocytes, Cytotoxic (immunology), Transcription, Genetic, Transfection, Viral Envelope Proteins (genetics), Viral Matrix Proteins, Viral Proteins (genetics).
- MESH :
- chemical , genetics : Viral Envelope Proteins, Viral Proteins.
- chemical , metabolism : DNA.
- genetics : Influenza A virus, Simian virus 40.
- immunology : Influenza A virus, L Cells, T-Lymphocytes, Cytotoxic.
- metabolism : L Cells.
- Teeft :
- Affinity chromatography, Animals, Antibody molecules, Antrbody, Askonas, Assay, Bacterial gene, Berg, Binding affinity, Binding characteristics, Braciale, Brownlee, Cdna clone, Cell, Cell clone, Cell clones, Cell clones lha6, Cell control, Cell growth factors, Cell recognition, Cell response, Cell surface, Cells cotransfected, Cells transfected, Cells, Cultured, Clone, Complete medium, Cotransfected, Crossreactive, Crossreactrve cytotoxic, Cultures lysed, Cytofluorograf, Cytotoxic, DNA Restriction Enzymes, Expenmental procedures, Experimental procedures, Expression vector, Female, First layer, First panel, Fluorescence profiles, Fourth panel, Further work, Gene, Gene copies, Gene transfer, Growth factors, Hemagglutinin, Hemagglutinin expression, Hemagglutinin gene, Hemagglutinin molecule, Hemagglutinin molecules, Hemagglutinin transfected, Hemagglutinrn gene, High levels, Human influenza, Immunoenzyme Techniques, Immunofluorescence, Immunoperoxidase staining, Indirect immunofluorescence, Indirect immunoperoxidase staining, Indirect rmmunofluorescence, Infectious allantoic flurd, Influenza, Influenza nucleoprotein, Influenza nucleoprotern, Influenza viruses, Intranasal infection, Lha6, Lha6 cells, Lha8, Limitrng dilution, Lysed, Lysis, Medium release, Mice, Mice, Inbred Strains, Molecule, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Mouse strain, Negative control, Nucleoprotein, Nucleoprotein gene, Original clone, Ortho, Ortho cytofluorograf, Plasmid, Plasmids, Polyclonal, Positive cells, Protein expression, Ptknp2, Recombinant, Release assay, Restimulated, Right panel, Room temperature, Sambrook, Scatchard, Scatchard analysis, Second panel, Skehel, Specific cytotoxic, Specific lysis, Specrfic cytotoxic, Spleen cells, Standard methods, Subclones, Subpopulation, Target cells, Third panel, Tkneo gene, Townsend, Transcription, Genetic, Transfected, Transfected cells, Transfected clone, Transfected mouse, Transfection, Triton release, Unpublished data, Upper section, Vaccine, Viral, Viral Matrix Proteins, Viral glycoproteins, Virus, Virus crossreactive, Wrth.
Abstract
Abstract: L cells expressing either the A/NT/60/68 nucleoprotein or the A/PR/8/34 (H1) hemagglutinin by DNA mediated gene transfer were used to investigate recognition by influenza A specific cytotoxic T lymphocytes (CTL). A subpopulation of CTL that recognized the H1 hemagglutinin was detected in mice primed with either A/PR/8/34 (H1N1) or A/JAP/305/57 (H2N2) influenza viruses. However, neither CTL from mice primed with A/NT/60/68 (H3N2) nor the recombinant virus X31 (H3N2) showed any activity on L cells expressing H1. These results showed that the majority of fully crossreactive CTL do not recognize the hemagglutinin molecule. A comparison between nucleoprotein and hemagglutinin transfected L cells reveals the nucleoprotein as the major target for CTL that are crossreactive on the three pandemic strains of human influenza A virus.
Url:
DOI: 10.1016/0092-8674(84)90187-9
Affiliations:
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Le document en format XML
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<term>Cells, Cultured</term>
<term>DNA (metabolism)</term>
<term>DNA Restriction Enzymes</term>
<term>Female</term>
<term>Immunoenzyme Techniques</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza A virus (immunology)</term>
<term>L Cells (immunology)</term>
<term>L Cells (metabolism)</term>
<term>Mice</term>
<term>Mice, Inbred Strains</term>
<term>Plasmids</term>
<term>Simian virus 40 (genetics)</term>
<term>T-Lymphocytes, Cytotoxic (immunology)</term>
<term>Transcription, Genetic</term>
<term>Transfection</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Matrix Proteins</term>
<term>Viral Proteins (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (métabolisme)</term>
<term>Animaux</term>
<term>Cellules cultivées</term>
<term>DNA restriction enzymes</term>
<term>Femelle</term>
<term>Lignées consanguines de souris</term>
<term>Lymphocytes T cytotoxiques (immunologie)</term>
<term>Plasmides</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de la matrice virale</term>
<term>Protéines virales (génétique)</term>
<term>Souris</term>
<term>Techniques immunoenzymatiques</term>
<term>Transcription génétique</term>
<term>Transfection</term>
<term>Virus de la grippe A (génétique)</term>
<term>Virus de la grippe A (immunologie)</term>
<term>Virus simien 40 (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Envelope Proteins</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
<term>Simian virus 40</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de l'enveloppe virale</term>
<term>Protéines virales</term>
<term>Virus de la grippe A</term>
<term>Virus simien 40</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term>
<term>L Cells</term>
<term>T-Lymphocytes, Cytotoxic</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>L Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Affinity chromatography</term>
<term>Animals</term>
<term>Antibody molecules</term>
<term>Antrbody</term>
<term>Askonas</term>
<term>Assay</term>
<term>Bacterial gene</term>
<term>Berg</term>
<term>Binding affinity</term>
<term>Binding characteristics</term>
<term>Braciale</term>
<term>Brownlee</term>
<term>Cdna clone</term>
<term>Cell</term>
<term>Cell clone</term>
<term>Cell clones</term>
<term>Cell clones lha6</term>
<term>Cell control</term>
<term>Cell growth factors</term>
<term>Cell recognition</term>
<term>Cell response</term>
<term>Cell surface</term>
<term>Cells cotransfected</term>
<term>Cells transfected</term>
<term>Cells, Cultured</term>
<term>Clone</term>
<term>Complete medium</term>
<term>Cotransfected</term>
<term>Crossreactive</term>
<term>Crossreactrve cytotoxic</term>
<term>Cultures lysed</term>
<term>Cytofluorograf</term>
<term>Cytotoxic</term>
<term>DNA Restriction Enzymes</term>
<term>Expenmental procedures</term>
<term>Experimental procedures</term>
<term>Expression vector</term>
<term>Female</term>
<term>First layer</term>
<term>First panel</term>
<term>Fluorescence profiles</term>
<term>Fourth panel</term>
<term>Further work</term>
<term>Gene</term>
<term>Gene copies</term>
<term>Gene transfer</term>
<term>Growth factors</term>
<term>Hemagglutinin</term>
<term>Hemagglutinin expression</term>
<term>Hemagglutinin gene</term>
<term>Hemagglutinin molecule</term>
<term>Hemagglutinin molecules</term>
<term>Hemagglutinin transfected</term>
<term>Hemagglutinrn gene</term>
<term>High levels</term>
<term>Human influenza</term>
<term>Immunoenzyme Techniques</term>
<term>Immunofluorescence</term>
<term>Immunoperoxidase staining</term>
<term>Indirect immunofluorescence</term>
<term>Indirect immunoperoxidase staining</term>
<term>Indirect rmmunofluorescence</term>
<term>Infectious allantoic flurd</term>
<term>Influenza</term>
<term>Influenza nucleoprotein</term>
<term>Influenza nucleoprotern</term>
<term>Influenza viruses</term>
<term>Intranasal infection</term>
<term>Lha6</term>
<term>Lha6 cells</term>
<term>Lha8</term>
<term>Limitrng dilution</term>
<term>Lysed</term>
<term>Lysis</term>
<term>Medium release</term>
<term>Mice</term>
<term>Mice, Inbred Strains</term>
<term>Molecule</term>
<term>Monoclonal</term>
<term>Monoclonal antibodies</term>
<term>Monoclonal antibody</term>
<term>Mouse strain</term>
<term>Negative control</term>
<term>Nucleoprotein</term>
<term>Nucleoprotein gene</term>
<term>Original clone</term>
<term>Ortho</term>
<term>Ortho cytofluorograf</term>
<term>Plasmid</term>
<term>Plasmids</term>
<term>Polyclonal</term>
<term>Positive cells</term>
<term>Protein expression</term>
<term>Ptknp2</term>
<term>Recombinant</term>
<term>Release assay</term>
<term>Restimulated</term>
<term>Right panel</term>
<term>Room temperature</term>
<term>Sambrook</term>
<term>Scatchard</term>
<term>Scatchard analysis</term>
<term>Second panel</term>
<term>Skehel</term>
<term>Specific cytotoxic</term>
<term>Specific lysis</term>
<term>Specrfic cytotoxic</term>
<term>Spleen cells</term>
<term>Standard methods</term>
<term>Subclones</term>
<term>Subpopulation</term>
<term>Target cells</term>
<term>Third panel</term>
<term>Tkneo gene</term>
<term>Townsend</term>
<term>Transcription, Genetic</term>
<term>Transfected</term>
<term>Transfected cells</term>
<term>Transfected clone</term>
<term>Transfected mouse</term>
<term>Transfection</term>
<term>Triton release</term>
<term>Unpublished data</term>
<term>Upper section</term>
<term>Vaccine</term>
<term>Viral</term>
<term>Viral Matrix Proteins</term>
<term>Viral glycoproteins</term>
<term>Virus</term>
<term>Virus crossreactive</term>
<term>Wrth</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules cultivées</term>
<term>DNA restriction enzymes</term>
<term>Femelle</term>
<term>Lignées consanguines de souris</term>
<term>Plasmides</term>
<term>Protéines de la matrice virale</term>
<term>Souris</term>
<term>Techniques immunoenzymatiques</term>
<term>Transcription génétique</term>
<term>Transfection</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Abstract: L cells expressing either the A/NT/60/68 nucleoprotein or the A/PR/8/34 (H1) hemagglutinin by DNA mediated gene transfer were used to investigate recognition by influenza A specific cytotoxic T lymphocytes (CTL). A subpopulation of CTL that recognized the H1 hemagglutinin was detected in mice primed with either A/PR/8/34 (H1N1) or A/JAP/305/57 (H2N2) influenza viruses. However, neither CTL from mice primed with A/NT/60/68 (H3N2) nor the recombinant virus X31 (H3N2) showed any activity on L cells expressing H1. These results showed that the majority of fully crossreactive CTL do not recognize the hemagglutinin molecule. A comparison between nucleoprotein and hemagglutinin transfected L cells reveals the nucleoprotein as the major target for CTL that are crossreactive on the three pandemic strains of human influenza A virus.</div>
</front>
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<affiliations><list><country><li>Royaume-Uni</li>
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<tree><country name="Royaume-Uni"><noRegion><name sortKey="Townsend, A R M" sort="Townsend, A R M" uniqKey="Townsend A" first="A. R. M." last="Townsend">A. R. M. Townsend</name>
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<name sortKey="Brownlee, G G" sort="Brownlee, G G" uniqKey="Brownlee G" first="G. G." last="Brownlee">G. G. Brownlee</name>
<name sortKey="Carter, N P" sort="Carter, N P" uniqKey="Carter N" first="N. P." last="Carter">N. P. Carter</name>
<name sortKey="Huddleston, J A" sort="Huddleston, J A" uniqKey="Huddleston J" first="J. A." last="Huddleston">J. A. Huddleston</name>
<name sortKey="Mcmichael, A J" sort="Mcmichael, A J" uniqKey="Mcmichael A" first="A. J." last="Mcmichael">A. J. Mcmichael</name>
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